Fig. 3: MBONs-β’2mp activity peaks after short training.
From: A neural circuit linking learning and sleep in Drosophila long-term memory
a Neural skeletons derived from the FIB-SEM Drosophila brain volume. b Mean GCaMP6s response (ΔF/F) traces (±SEM) in vFBs upon optogenetic MBONs-β’2mp activation (red bar). n = 5 flies per group, Pleft = 0.0303, Pright = 0.0071 for H0 ΔF/F = 0, two-sided Student T test. c. Normalized mean luminescence traces (±SEM). d. Mean normalized luminescence in the 1–3 h time period (TP1-3) after training in c. n0 h = 47, n1 h = 38, n2 h = 39, n4 h = 44, n6 h = 38, P1 h = 0.2769, P2 h = 0.0056, P4 h = 0.1043, P6 h = 0.8331 for H0 N. Lum.exp = N. Lum.nai, and P = 8.6e−4 for H0 N. Lum.2 h = N. Lum.6 h, two-sided Student T test. e Mean sleep traces (±SEM) of ΔSleep (exp-nai) upon MBONs-β’2mp silencing. f Mean ΔSleep (exp-nai) in TP1-3 in e. n1 = 54, n2 = 49, n3 = 39, n4 = 42, P1 = 0.6073, P2 = 0.0207, P3 = 0.3553, P4 = 0.3451 for H0 ΔSleep = 0, two-sided Wilcoxon Signed Rank test, and P12 = 0.0273, P34 = 0.3559 for H0 ΔSleep20 °C = ΔSleep30 °C, two-sided Wilcoxon Rank Sum test. g LTM, shown as SI [%] upon MBONs-β’2mp silencing. n1 = 53 and 52, n2 = 59 and 61, n3 = 52 and 57, n4 = 72 and 59 for naïve and trained group respectively, P1 = 0.2188, P2 = 0.0019, P3 = 0.4019, P4 = 0.2663 for H0 SI = 0, and P12 = 0.0376, P34 = 0.8575 for H0 SIexp = SIctrl, two-sided Permutation test. h Mean ΔSleep (exp-nai) in TP1-3 upon MBONs-β’2mp activation. n1 = 40, n2 = 40, n3 = 40, n4 = 38, P1 = 0.0025, P2 = 0.6374, P3 = 0.0014, P4 = 1.2e−5 for H0 ΔSleep = 0, two-sided Wilcoxon Signed Rank test, and P12 = 0.0023, P34 = 0.8768 for H0 ΔSleepnoact = ΔSleepact, two-sided Wilcoxon Rank Sum test. i LTM, shown as SI [%], upon MBONs-β’2 mp activation. n1 = 56 and 57, n2 = 57 and 56, n3 = 30 and 40, n4 = 33 and 37 for naïve and trained group respectively, P1 = 1.0e−5, P2 = 0.0517, P3 = 4.8e−4, P4 = 1.0e−5 for H0 SI = 0, and P12 = 0.0403, P34 = 0.9542 for H0 SIexp = SIctrl, two-sided Permutation test. Full genotypes and data analysis details in Supplementary Tables 1–3. Source data are provided as a Source Data file. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. n represents independent fly samples with assays repeated at least 3 times (d, f, g, h and i). Box plots represent median and IQR, whiskers extend to lower and upper adjacent values and red crosses for outliers (d, f and h).
