Fig. 6: Fe-dependent callose deposition in the phloem via reactive oxygen species (ROS) in response to NH4+.
From: Phloem iron remodels root development in response to ammonium as the major nitrogen source
a Callose detection in primary roots of Col-0, lpr2-1, and complementation line COM#7 seedlings by aniline blue staining. b Radial section (top) and close-up view (bottom) of aniline blue staining in primary roots of Col-0 seedlings grown in Fesuff NH4+ medium. c Fluorescence co-localization of GFP-sporamin and aniline blue staining in pSUC2::GFP-sporamin roots. d ROS scavengers abolished the NH4+-induced callose deposition. Four-day-old seedlings of the indicated genotypes were transferred to NO3− or NH4+ medium with Fesuff (100 µM) or Felow (10 µM) with or without the indicated ROS scavenger. Each experiment was repeated independently three times with similar results, and a representative experiment is shown.
