Fig. 5: Transcriptional profiling of mutant and control N transfected cells.

Calu-3 cells were transfected with plasmids expressing the full-length N-control and N-mutant protein along with mock control (4 biological replicates per condition). 48-h post-transfection total RNA was isolated and subjected to RNA-sequencing using illumina NovaSeq 6000 platform. a Heatmap shows normalized expression of top significantly differentially expressed genes in N-mutant and N-control conditions (adj p-value <0.05 and log2 fold-change cutoff ≥1). Genes enriched in interferon and immune-related processes are overexpressed in the N-mutant transfected cells. The heatmap was generated by the visualization module in the NetworkAnalyst. b Plot showing comparison of fold-changes for all DE genes in N-mutant and N-control conditions. Differentially expressed genes display higher up-regulation in the N-mutant condition (as orange dots that represent common up-regulated genes are skewed towards the lower half of the diagonal). c Venn diagram shows the common and unique up-regulated genes in both conditions. d GO-enrichment analysis (top 15 pathways based on p-value and FDR are shown) of up-regulated genes. The enriched GO BP (Biological Processes) term is related to defense and interferon response. The enriched terms display an interconnected network with overlapping gene sets (from the list). Each node represents an enriched term and colored by its p-value from red to blue in ascending order (red shows the smallest p-value) as shown in Supplementary Data 6. The size of each node corresponds to number of linked genes from the list.