Fig. 1: Discovery and characterization of B. atropi infection in O. tipulae.
From: Bacterial filamentation as a mechanism for cell-to-cell spread within an animal host
a A multitude of coccobacilli (arrowheads) inside the intestinal cells (in) of O. tipulae strain JU1501. The intestinal lumen (lu) and pseudocoelom (pc) are indicated. b, c FISH using a universal bacterial 16S rRNA probe EUB338. Different animals shown from the same plate. Scale bars are 20 μm. d Phylogenomic tree of sequenced Bordetella and Achromobacter spp. with outgroup Alcaligenes faecalis. Branch lengths are the number of substitutions per site and branch points indicate percentage of trees with clustering of associated taxa. e Lifespan of JU1501 infected or uninfected with B. atropi, n = 20 animals in 3 independent experiments. Points represent mean values and error bars represent standard deviation (SD). f Broodsizes of JU1501, **** is p < 0.0001 by unpaired, two-tailed t-test with Welch’s correction, n = 20 animals in 2 independent experiments. Error bars indicate SD and each point represents number of progeny from a single animal. g Pulse-chase infection time course, n = 857 animals binned over 2 independent experiments, error bars represent SD. Representative infection phenotypes are shown. Scale bars are 20 μm. h Histogram of in vivo filament lengths at 34 hpi.
