Fig. 3: B. atropi filaments localize with intestinal intracellular staining.
From: Bacterial filamentation as a mechanism for cell-to-cell spread within an animal host
a Representative confocal image of FISH-stained animals infected with B. atropi and stained with the cytoplasmic dye, CFSE, which strongly stains the intestinal cell cytoplasm. b Orthogonal view of white-boxed region in a. c Higher magnification of red-boxed region in a showing CFSE-cleared areas (white arrowheads) are overlapped by signals from 16S probe specific-FISH to B. atropi. d Representative image of animals stained with phalloidin. e Higher magnification of region indicated by white box in d showing B. atropi presence in intestinal cells outlined by basolateral and apical actin filaments (mu, body wall muscle is indicated). f Line profile analysis at the plane indicated by yellow lines in d showing localization of tdTomato (B. atropi) in-between Alexa488-phalloidin (actin). g Quantification of bacterial localization from the experiment conducted in d, with a total of n = 60 animals containing N = 916 infection events analyzed in 2 independent experiments. Means are shown and error bars represent SD. h A phalloidin-stained animal with a fragment of filament pushing through intestinal lateral actin. i Inset indicated in h. Scale bars are 10 μm.
