Fig. 2: Microbial taxonomic composition of capybara gut microbiome.

a Relative phyla abundance based on 16S rRNA gene target sequencing (16S), 16S rRNA recovered from metagenome (16S_MG), whole metagenome (MG) and metatranscriptome (MT) reads. b Ratio of average relative phyla abundance (Transcripts Per Million - TPM) of metatranscriptome to metagenome (MT/MG), expressed in a log2 scale. Bars marked with an asterisk (*) are significantly different from MT/MG = 1 (two-tailed t-test, P-value with false discovery rate correction for multiple tests, 0.05 was used as the threshold of significance level). The data represent the average of three independent experiments (n = 3), using fresh samples collected from the cecum and recto of three wild animals by abdominal surgical procedure. c Phylogenetic tree of the 79 recovered metagenome-assembled genomes (MAGs) from capybara microbiota, including related genomes publicly available. The capybara microbiota taxonomic novelties and some reference genomes are highlighted with red and blue circles, respectively. Source data are provided as a source data file.