Fig. 3: Stk11^ec−/− mice develop a myeloid proliferative disorder with depressed DC differentiation.

a, b Flow cytometry analysis of spleen, lymph node (LN), and thymus cells isolated from WT or Stk11^ec−/− mice and stained for CD11c, MHC II, and B220. Classical DCs (cDCs) were defined as CD11c⁺ MHC II⁺, plasmacytoid DCs (pDCs) were defined as CD11c^low B220⁺. Bar graph summarizes cell number of cDC and pDC in spleen (n = 11–13 each group), LN (n = 11 each group), and thymus (n = 4–5 each group) (12-weeks-old, mixed-gender). ^†P = 0.006; ^*P < 0.001 versus WT by nonparametric Mann–Whitney U test (two-sided). c Flow cytometry analysis of spleen cells isolated from WT or Stk11^ec−/− mice and stained for Lin (CD3, CD19, CD49b, Ly6G), B220, F4/80, and CD11b. Macrophages were defined as Lin^- B220⁻ F4/80⁺ CD11b⁺. Bar graph summarizes cell number of macrophage in spleen (n = 11–13 each group), LN (n = 4–5 each group), and thymus (n = 4–6 each group) (12-weeks-old, mixed-gender). ^†P = 0.003; ^*P < 0.001 versus WT by nonparametric Mann–Whitney U test (two-sided). d Flow cytometry analysis of bone marrow cells isolated from WT or Stk11^ec−/− mice and stained for Lin (CD3, CD19, Ter119, NK1.1, B220), CD45, CD11c, MHC II, SIRP-α, and Flt3. Committed precursors of cDC (pre-cDC) were defined as Lin^- CD45⁺ CD11c⁺ MHC II⁻ SIRP-^αint Flt3⁺. Bar graph summarizes cell number of pre-cDC in bone marrow (12-weeks-old, mixed-gender, n = 12–13 each group). *P < 0.001 versus WT by nonparametric Mann–Whitney U test (two-sided). e Flow cytometry analysis of bone marrow cells isolated from WT or Stk11^ec−/− mice and stained for Lin, c-Kit, CD115, CX3CR1, and Flt3. Macrophages and DC precursors (MDPs) were defined as Lin⁻ c-Kit^high CD115⁺ CX3CR1⁺ Flt3⁺. Common DC precursors (CDPs) were defined as Lin⁻ c-Kit^low CD115⁺ Flt3⁺. Bar graph summarizes cell number of MDP/CDP in bone marrow (12-weeks-old, mixed-gender, n = 9–13 each group). ^†P = 0.002; ^*P < 0.001 versus WT by nonparametric Mann–Whitney U test (two-sided).