Fig. 5: Isolation of trimer-specific monoclonal antibodies from an animal (272.8) with potent neutralization activity against BG505.T332N post vaccinations with DNA-encoded BG505.MD39. | Nature Communications

Fig. 5: Isolation of trimer-specific monoclonal antibodies from an animal (272.8) with potent neutralization activity against BG505.T332N post vaccinations with DNA-encoded BG505.MD39.

From: Induction of tier-2 neutralizing antibodies in mice with a DNA-encoded HIV envelope native like trimer

Fig. 5

a Vaccination and tissue collection scheme for 272.8. b Trimer-specific binding antibody responses in 272.8 2 weeks post the final vaccination. c Autologous BG505.T332N neutralizing activity induced in 272.8 2 weeks post the final vaccination. d Gating strategy used to isolate antigen-specific CD19 + IgD− IgM− BG505.MD39-Tetramer-FITC + BG505.MD39-Tetramer-PE + B-cells from spleen and lymph nodes of 272.8 2 weeks post the final vaccination. e Characteristics of isolated murine monoclonal antibodies in terms of germline VH and VL gene usage.

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