Fig. 2: Distinct immune microenvironments associate with immune diversity and clinical outcomes in HGSC.
From: Single-cell tumor-immune microenvironment of BRCA1/2 mutated high-grade serous ovarian cancer
a A hierarchical clustering heatmap of immune subtype proportions out of all immune cells, annotated with immune diversity (immune SDI), and clinical data including HR status and PFI. The barplot annotations for the columns and rows represent the total number of cells per each immune cell subtype and the number of the immune cells in total per patient, respectively. b Boxplot showing the combined proportion of IBA1 + , CD163 + and IBA1 + CD163 + macrophages as a proportion of all cells, stratified by HR status. c Boxplot showing the increased proportion of CD11c + antigen-presenting cells as well as d increased proportion of IBA1 + CD163 + CD11c + macrophages as a proportion of all immune cells in HRwt as compared to BRCA1/2mut tumors e Boxplot showing increased ratio of CD8 + T-cells to IBA1 + CD163 + CD11c + macrophages in BRCA1/2mut as compared to HRwt tumors. f Boxplot showing higher immune diversity in HRwt as compared to BRCA1/2mut tumors. The differences between the boxplot groups were calculated by two-tailed Wilcoxon rank-sum test. The black horizontal lines represent the sample medians, the boxes extend from first to third quartile and whiskers indicate the values at 1.5 times the interquartile range. Individual dots represent values per each tumor (n = 31 BRCA1/2mut, n = 13 HRwt). g Kaplan-Meier graphs for PFI show improved PFI for high CD8 + T-cell proportion in all tumors pooled as well as h for high CD4 + T-cell proportion in patients with BRCA1/2mut tumors. Proportions were calculated out of all cells, and the median value was used to distinguish high and low groups. Number of patients at risk is shown at the bottom of each Kaplan-Meier graph. The comparisons between the groups were performed using the log-rank test. i Scatter plots with lowess regression show negative correlation of immune diversity and the proportion of IBA1 + CD163 + macrophages in all HR groups and j a positive correlation of immune diversity and the proportion of IBA1 + CD163 + CD11c + macrophages in BRCA1/2mut tumors. Proportions were calculated out of immune cells and Spearman correlation coefficients and their p-values are shown (no FDR adjustment). HR groups include BRCA1/2mut (n = 31, blue), HRwt (n = 13, red) tumors and all tumors pooled (n = 44, black). Source data are provided with this paper.
