Fig. 4: HR-genotypes show different patterns of cell type co-occurrence with CD8 + T-cells and CD4 + T-cells.
From: Single-cell tumor-immune microenvironment of BRCA1/2 mutated high-grade serous ovarian cancer
a A hierarchical clustering heatmap of all cell proportions shows enrichment of HRwt tumors in the cluster characterized by stromal metaclusters. Annotations include the HR status, PFI group, as well as the global cell type of the metaclusters. b A Spearman correlation dot plot between tumor metaclusters, immune subtypes and stromal metaclusters as proportions out of all cells, divided by HR status (n = 31 BRCA1/2mut, n = 13 HRwt). Dots in white background and light-red background represent correlations in BRCA1/2mut and HRwt tumors, respectively. Dots shaded red represent positive correlations while dots shaded blue represent negative correlations. Black lines separate the tumor, immune and stromal cell columns and rows. Blue dashed boxes highlight the interactions that CD4 + T-cells and CD8 + T-cells have with other cell types. Correlations with a p-value < 0.05 are shown and those passing FDR 0.1 are highlighted with black squares. c Principal component feature projections for BRCA1/2mut (n = 31) tumors and d for HRwt (n = 13) tumors. 12 cell types with the highest contributions to PC1 and PC2 are shown for each plot. S = stroma, macs = macrophages. The more intense the color of the cell type, the coefficient is. Colors represent global cell types (grey = tumor, yellow = stroma, purple = immune). e Plot of patients with BRCA1/2mut (n = 31) tumors and f plot of patients with HRwt (n = 13) tumors projected onto their first two principal components. Patients are annotated as having a long or short PFI and colored by light blue or dark blue, respectively. Ellipsoids show the 95% confidence intervals for the PFI groups. Source data are provided with the paper.
