Fig. 2: Comparative metabolomics of CA-fed hacl-1 mutants. | Nature Communications

Fig. 2: Comparative metabolomics of CA-fed hacl-1 mutants.

From: Comparative metabolomics with Metaboseek reveals functions of a conserved fat metabolism pathway in C. elegans

Fig. 2

a Conservation of peroxisomal α-oxidation in C. elegans and humans. b CA-feeding experiment. C. elegans larvae were supplemented with CA, (6) followed by comparative analysis with Metaboseek. c hacl-1(tm6725) mutants harbor a genomic deletion (red line) spanning the indicated exon splice junction, orange rectangles represent exons, black lines represent introns. d Representative shunt metabolites accumulating in hacl-1 following CA supplementation. The majority of shunt metabolites fragment between the carbonyl- and α-carbon during MS/MS, which is characteristic of α-hydroxy fatty acids. e HPLC-MS (negative ion) EIC for hacl-1-enriched feature with m/z 185.1182, corresponding to 7 (arrow). Its MS/MS spectrum reveals a product ion at m/z 139.1123, corresponding to neutral loss of formic acid.

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