Fig. 3: Endogenous metabolites accumulating in hacl-1 mutant larvae. | Nature Communications

Fig. 3: Endogenous metabolites accumulating in hacl-1 mutant larvae.

From: Comparative metabolomics with Metaboseek reveals functions of a conserved fat metabolism pathway in C. elegans

Fig. 3

a MS/MS network highlighting differential abundance in hacl-1 mutants relative to WT. Subnetworks of interest (orange boxes) are shown enlarged. b Quantification of endogenous metabolites ten-fold or more enriched in hacl-1 larvae relative to WT at p < 0.05 as calculated by unpaired two-sided t-test, unadjusted for number of comparisons. Metabolites in red produce an MS/MS product ion with m/z 72.993. Data represent five independent experiments and bars means ± standard deviation. c Representative HPLC-MS (negative ion) EICs and MS/MS spectra for most abundant differential metabolites in b bemeth#3 (11) and bemeth#2 (12). d Overview of synthetic scheme to afford bemeth#2 (see Methods for details). e Comparison of HPLC-MS (negative ion) EICs for synthetic diastereomers of bemeth#2 (12) and the corresponding metabolites in exo-metabolome extracts from WT and hacl-1 larvae. Source data are provided as a Source Data file.

Back to article page