Fig. 2: TARP loop interaction sites on the LBDs.
From: Mechanisms underlying TARP modulation of the GluA1/2-γ8 AMPA receptor
a Schematic of TARP γ8, showing transmembrane helices (TM1–4), the 5-stranded beta sheet and the three attached loop elements. b cryo-EM maps obtained by focussed processing, showing state-dependent interaction of the β1-loop with the GluA1 and GluA2 LBDs (indicated by arrows). c Left panel: β1-loop contacts on the LBDs obtained by MD simulations reveal two patches (yellow) that closely resemble the interactions sites described in (b). Right panel: environment of patch 1 on the GluA2 LBD (red), and the GluA1 LBD (blue). d The TARP-γ8 β1 loop mediates slowing of recovery from desensitization. Functional interaction between GluA2 D1 lobe and γ8 β1-loop may contribute to the stabilization of the desensitized state. Desensitization recovery tau (ms): A1/A2_γ8, 92.79 ± 4.09 (mean ± SEM), n = 19; A1/A2_γ2, 74.01 ± 3.66, n = 10, (*p = 0.0210); A1/A2_γ8 (γ2 β1), 75.38 ± 2.62, n = 10, (*p = 0.0381); A1/A2_γ8 (γ2 ex2), 105.2 ± 8.20, n = 5 (ns, p = 0.5056); A1/A2, 53.62 ± 4.90, n = 4, (***p = 0.0002); A1/A2_γ8 glyco, 134.2 ± 5.51, n = 11, (***p < 0.0001); A1 glyco/A2_γ8, 95.10 ± 8.48, n = 6, (ns, p = 0.9996). One-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test (against A1/A2_γ8 as control), p < 0.0001. Source data are provided as a Source data file.
