Fig. 1: Particulate β-glucan traffics to the pancreas in a dectin-1 dependent manner.

a DTAF-WGP was injected I.P. and 3 days later different tissues (n = 3) were harvested and assessed for the presence of the DTAF-WGP by flow cytometry. Representative dot plots and summarized data are shown. *p = 0.032, **p = 0.0057, ****p < 0.0001. b WGP was labeled with ⁸⁹Zr-WGP or c peritoneal macrophages were incubated with ⁸⁹Zr-WGP and washed, followed by I.P. injection. PET/CT imaging displays the trafficking of the ⁸⁹Zr-WGP after 48 h. Organs were individually assessed for radioactivity following a necroscopy using a gamma counter. In c, significance is reported as compared to the pancreas (n = 3). ****p < 0.0001. d Dectin-1^−/− mice or WT mice were injected with DTAF-WGP and the accumulation of DTAF-WGP in the pancreas was assessed by flow cytometry (WT PBS n = 3, WT WGP n = 4, KO + PBS n = 3, KO + WGP n = 4). e Dectin-1^−/− mice or WT mice (n = 4) were injected with ⁸⁹Zr-WGP and 48 h later a PET/CT was used to assess the amount in the pancreas. The signal was quantified by reporting the % of injected dose (%ID) in the pancreas. *p = 0.046. A one-way ANOVA with Tukey’s multiple comparisons was used in a–d, and an unpaired, two-tailed student’s t-test was used in e. Data were represented as mean ± SEM. ns not significant; Each sample represents a biologically independent animal obtained over a single independent experiment which was repeated at least twice for verification of results.