Fig. 3: Single-cell RNA-seq showing the immune cell phenotype 3 and 7 days following IP WGP. | Nature Communications

Fig. 3: Single-cell RNA-seq showing the immune cell phenotype 3 and 7 days following IP WGP.

From: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression

Fig. 3: Single-cell RNA-seq showing the immune cell phenotype 3 and 7 days following IP WGP.

CD45+ cells from the pancreas of mice treated with PBS or WGP 7 days and 3 days prior were sorted and scRNA-Seq was performed. a Two-dimensional UMAP representation of 11,132 cells aggregated from the three experimental samples with 20 unique clusters resulting from k-nearest neighbors and Louvain algorithms. b Heatmap of expression of aggregated marker genes for all clusters. c Bar graphs showing the relative frequency of cells in each cluster across samples. d UMAP dimension reduction of PBS (blue), 3-day WGP (green), and 7-day WGP (red) samples shown individually. The portion of the UMAP representing myeloid cells is highlighted. e Single-cell gene expression of CSF1R. f Single-cell expression distributions across clusters identified as myeloid cells for select genes related to myeloid phenotyping. g Dot plot of the top 12 enriched genes in clusters 3, 4, 5, and 10 showing the average expression level and percentage of cells expressing select genes. h Dot plots showing the enrichment of selected genes associated with pro-inflammatory (green) and anti-inflammatory (red) immune responses across clusters 3, 4, 5, and 10. In g and h, the average expression level is displayed as z-scores computed across the four clusters for individual genes.

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