Fig. 1: Automated high-content profiling platform demonstrates reproducibility across batches. | Nature Communications

Fig. 1: Automated high-content profiling platform demonstrates reproducibility across batches.

From: Integrating deep learning and unbiased automated high-content screening to identify complex disease signatures in human fibroblasts

Fig. 1

a Workflow overview. b Overview of automated experimental pipeline. Scale bar: 35 μm. Running this pipeline yielded low variation across batches in: c well-level cell count; d well-level foreground staining intensity distribution per channel and plate; and e well-level image focus across the endoplasmic reticulum (ER) channel per plate, for n = 96 wells per plate. Box plot components are: horizontal line, median; box, interquartile range; whiskers, 1.5× interquartile range; black squares, outliers.

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