Fig. 7: DEER spectra of unliganded BG505 trimer compared to Ab1303-bound and Ab1573-bound BG505 trimer. | Nature Communications

Fig. 7: DEER spectra of unliganded BG505 trimer compared to Ab1303-bound and Ab1573-bound BG505 trimer.

From: Neutralizing antibodies induced in immunized macaques recognize the CD4-binding site on an occluded-open HIV-1 envelope trimer

Fig. 7

a Left: Triangles showing distances between residue 174 Cα atoms on cartoon representations of Env trimers seen from the top. Right: Inter-protomer distances between Cα atoms for V1V2 residue 174 in different Env conformational states measured using coordinates from the indicated structures (BG505 trimer: PDB 5T3Z; Ab1303-BG505 and Ab-1573-BG505: this study; b12-B41: PDB 5VN8; CD4-B41: PDB 5VN3). b, c Left: Distance distributions for spin labels attached to V1V2 residue 174 in unliganded BG505 Env (solid black), Ab1303-bound BG505 (green trace in b), and Ab1573-bound BG505 (teal trace in c). Vertical dashed lines indicate the inter-subunit distances measured from coordinates (a) for each site in structures of closed, unliganded Env (red line), Ab1303-bound Env (green dashed lines in b), Ab-1573-bound Env (teal dashed lines in c), and b12-bound Env (purple dashed line). Right: Legend for DEER spectra. The distance distributions smaller than 38 Å were boxed in light gray shades. Source data are provided as a Source Data file.

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