Fig. 8: Hematopoietic Grx1 deficiency promotes atherosclerosis in reproductively healthy female LDLR^-/- mice by enhancing recruitment of monocyte-derived macrophages into atherosclerotic lesions.

a Representative images of Oil Red O-stained aortas from female LDLR^-/-Grx1_Leuko^-/- mice (Grx1_Leuko^-/-) and age-matched female LDLR^-/- mice (WT) fed a HCD. b Quantification of aortic lesion area. n = 14 per group. Week 6: n = 10; Week 20: n = 14. c Representative images of Oil Red O-stained sections from the aorta roots of HCD-fed female LDLR^-/-Grx1_Leuko^-/- (Grx1_Leuko^-/-) and age-matched female LDLR^-/- mice (WT). Scale bar = 500 μm. d Quantitation of lesion area in the aortic root. Week 6: n = 10; Week 20: n = 14 per group. e Distribution of lesion size across a 500 μm segment of the aortic root beginning at the aortic valve. n = 14 mice per group. f Recruitment monocyte-derived macrophages into implanted Matrigel plugs loaded with. MCP-1. n = 14 mice per group. g Representative images of aortic root sections stained with a macrophage-specific antiserum against CD68. Scale bar = 500 μm. h Quantitation of macrophage content in atherosclerotic lesions in the aortic root. Week 6: n = 10; Week 20: n = 14 mice per group. All data are expressed as mean ± S.E. One-way ANOVA followed by Fisher’s Least Significance Difference test was used to compare the mean values between experimental groups. Source data are provided as a Source Data file.