Fig. 3: Transgenerational inheritance of lipid accumulation depends on fat-5, fat-6 and fat-7. | Nature Communications

Fig. 3: Transgenerational inheritance of lipid accumulation depends on fat-5, fat-6 and fat-7.

From: Histone H3K4me3 modification is a transgenerational epigenetic signal for lipid metabolism in Caenorhabditis elegans

Fig. 3

a Schematic presentation of the transcriptional network that regulates delta-9 desaturase gene. b–f Transgenerational inheritance test of lipid accumulation induced by HFD in fat-5 mutant (n ≥ 28 per condition) (b), fat-6 mutant (n ≥ 28 per condition) (c), fat-7 mutant (n ≥ 28 per condition) (d), fat-5; fat-6 double mutant (n ≥ 28 per condition) (e), and fat-5; fat-7 double mutant (n ≥ 30 per condition) (f) (mean ± SD; unpaired two-tailed Student’s t-test; ***p < 0.001, and ns: not significant). The same WT control was shown in a–f. g P0 worms were fed with or without HFD, and F1 progeny were exposed to fat-5 RNAi to determine the requirement of fat-5 in the F1 generation. h P0 animals were fed with or without HFD and subjected to fat-5 RNAi, and then lipid levels of F1 progeny were analyzed. For g and h (right panels), graph data are presented as mean ± SD, n ≥ 31 per condition, statistical analyses were performed by unpaired two-tailed Student’s t-test; ***p < 0.001, **p < 0.01, and ns: not significant, Source data are provided as a Source Data file.

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