Fig. 2: Blue light stimulation depolarizes membrane potential and inhibits secretion of PTH in parathyroid cells from patients with secondary hyperparathyroidism.

a Schematic showing lentivirus carrying the CMV-ChETA-eYFP construct. b Representative image of parathyroid cells expressing ChETA-eYFP. Scale bar = 50 μm. c Whole-cell patch-clamp was used to investigate the function of ChETA in human parathyroid cells. Scale bar = 20 μm. d Blue light stimulation induced membrane depolarization of ChETA-expressing parathyroid cells. e Change of depolarized membrane potential in the eYFP and ChETA groups (n = 10 cells). Two-tailed unpaired t-test, p < 0.0001. Values represent mean ± SEM. f Blue light stimulation induced ChETA depolarization spike trains. g Schematic diagram of PTH secretion at different time points after light stimulation of ChETA-expressing parathyroid cells. h A blue light-emitting diode (LED) was used to illuminate the cultured cells in 6-well plates. i Schematic showing the arrangement of the lamps in the LED constructed for light illumination. j Quantification of PTH was performed on the stimulated human parathyroid cells from SHPT patient 1 (n = 3 times per group). Two-tailed unpaired t-test, p values as indicated. Values represent mean ± SEM. k Quantification of PTH was performed on stimulated human parathyroid cells from SHPT patient 2 (n = 3 times per group). Two-tailed unpaired t-test, p values as indicated. Values represent mean ± SEM. l Quantification of PTH was performed on stimulated human parathyroid cells from patient 3 with SHPT (n = 3 times per group). Two-tailed unpaired t-test, p values as indicated. Values represent mean ± SEM. Source data are provided as a Source Data file.