Fig. 7: Optogenetic stimulation inhibits PTH secretion and promotes bone remodeling.
From: An optogenetic approach for regulating human parathyroid hormone secretion
a Schematic diagram of the establishment of a nude mouse PTG transplantation model. b Quantification of PTH levels in the ChETA and PTG groups before (B) and at 5, 15, and 30 min after light stimulation (n = 5 per group). Two-tailed unpaired t-test, p values as indicated. Values represent mean ± SEM. c MicroCT scanning was used to evaluate the structural changes of both cortical and trabecular bone in normal control, PTG and ChETA groups 28 days after blue light stimulation. Scale bar = 1 mm. d Quantification of bone mineral density (BMD) and bone volume (BV) of cortical bone from normal control, PTG, and ChETA groups (n = 5 per group). p = 0.003, p = 0.9183 for BMD; p = 0.0317, p = 0.9968 for BV. e Quantification of bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular number (TbN), and trabecular separation (Tb.Sp) of trabecular bone from normal control, PTG, and ChETA groups (n = 5 per group). f Alkaline phosphatase (ALP) staining of trabecular bone in the control, PTG, and ChETA groups. Scale bar = 150 μm. g Tartrate-resistant acid phosphatase (TRAP) staining in the control, PTG and ChETA groups. Scale bar = 150 μm. h Quantification of ALP-positive osteoblast per tissue volume (Ob/TV) in trabecular bones from control, PTG and ChETA groups (n = 6 per group). p = 0.0455, p = 0.002, respectively. i Quantification of the number of TRAP-positive osteoclasts per tissue volume (Oc/TV) in trabecular bones from control, PTG, and ChETA groups (n = 6 per group). p = 0.0441, p = 0.0004, respectively. j In vivo calcein labeling and calculation of mineral apposition rate (MAR) in cortical bone in the control, PTG and ChETA groups (n = 12 sections per group). p = 0.0016, p = 0.0214, respectively. Scale bar = 100 μm. k Quantification of serum Rankl level in the control, PTG, and ChETA group (n = 5 mice per group). p = 0.0287, p = 0.0344, respectively. l Quantification of serum osteoprotegrin (OPG) levels in the control, PTG and ChETA groups (n = 5 per group). All statistical tests in d–l used: One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test, p values as indicated. Values represent mean ± SEM. Source data are provided as a Source Data file.
