Fig. 7: l-Proline production using strains with transport engineering. | Nature Communications

Fig. 7: l-Proline production using strains with transport engineering.

From: CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

Fig. 7

a Effects of cgl2622 overexpression on l-proline production in 24-deep-well plates. Strain PRO-18 was constructed by adding a second copy of cgl2622 controlled by the derepressed Ptrc promoter in the chromosome of strain PRO-13. Data are presented as mean values +/− SD (n = 3 independent experiments). ***P = 0.0004, Student’s two-tailed t-test. b Fed-batch fermentation of strain PRO-18 in a 5 L bioreactor with 1 mg/mL biotin for a biotin-rich condition. c Fed-batch fermentation of strain PRO-18 in a 5 L bioreactor with 45 μg/mL biotin for a biotin-limited condition. d Fed-batch fermentation of strain PRO-19 (strain PRO-18 with cgl1270 (mscCG) deleted) in a 5 L bioreactor. Biotin was used at 45 μg/mL for a biotin-limited condition. Source data underlying Fig. 7 are provided as a Source Data file.

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