Fig. 2: Subtype-specific binding of anti-GluN1-GluN2B NMDA receptor antibodies.
From: Development and characterization of functional antibodies targeting NMDA receptors
a–d Purified IgG2 (panels a and c) or Fab2 (panels b and d) are mixed with GluN1b-GluN2B ATD (panels a–b) or GluN1b-GluN2A ATD (panels c–d) heterodimeric proteins and subjected to Superdex200 size-exclusion chromatography using tryptophan fluorescence (280 nm/330 nm = excitation/emission) as a detection method. Arrows indicate shifted peaks compared to non-mixed controls. e–h Equivalent experiments for IgG5 (panels e and g) and Fab5 (panels f and h) where GluN1b-GluN2B ATD (panels e–f) and GluN1b-GluN2A ATD (panels g–h) were mixed. The color code for chromatographs is shown on top of in each panel.
