Fig. 8: Fv2 binding and long-range interactions for bi-lobe closure of GluN2B ATD.
From: Development and characterization of functional antibodies targeting NMDA receptors
a GluN2B ATD R1 and R2 residues used as source and target nodes for computing optimal paths (surrounded by gray ovals). b–d Fv2 paths, involving GluN2B R1 residues 131-136 (panel b), GluN2B ATD R1 residues 103-106 (panel c) GluN2B Tyr282 at the R1–R2 hinge (panel d). e–g Fab2 paths transmitted through the R1-R2 linker residues 146-149 (panel e), 283-288 (panel f), 342-361 (panel g). h Fv2 paths that involve inter-subunit communication starting from GluN2B ATD R1 to GluN1b ATD R1, and into GluN2B ATD R2. i Same analysis for Fv5-ATD reveals one pathway that involves GluN1b-GluN2B contacts, although present in a very small percentage of the path ensemble. It is important to note that this path only involves a single point of contact on GluN1b, suggesting that correlated motion throughout GluN1b is specific to Fab2. All indicated residues are marked by large spheres.
