Fig. 2: Overexpression of KIRREL1 enhances Hippo signaling in HEK293A cells.
a Overexpression of KIRREL1 decreases the expression of TAZ protein and increases YAP phosphorylation. HEK293A cells were transfected with increasing amount of Myc-KIRREL1-HA construct and seeded at medium density (0.5 × 106 cells/well) in 12-well plate 24 h of post-transfection. Cells were harvested the next day and analyzed by western blot. b Left panel, overexpression of wild-type KIRREL1 decreases YAP-mediated GTIIC-GFP reporter activity in HEK293A GTIIC-GFPPEST Cas9 cells. Right panel, western blot showing KIRREL1 expression. c Overexpression of wild-type KIRREL1 decreases YAP target gene expression. The data represent mean ± SD, n = 4, error bars denote the SD between four biological replicates; Unpaired two-tailed t-test was used to determine the statistical significance, ***p value < 0.001. d Schematic of wild-type (WT) and ΔICD mutant of KIRREL1. e Overexpression of KIRREL1 inhibits YAP-responsive GTIIC-luciferase reporter activity. HEK293A cells were co-transfected with YAP and indicated constructs along with GTIIC-luciferase, and TK-Renilla luciferase, and subjected to dual luciferase assay. The data represent mean ± SD, n = 4, error bars denote the SD between four biological replicates; Unpaired two-tailed t-test was used to determine the statistical significance biological replicates, ***p value < 0.001, ns—0.3886. Source data for Fig. 2a–c, e are provided as Source Data file.
