Fig. 5: Chlamydia and HPV E6E7 coinfection regulates MMR distinctly at transcriptional and translational levels. | Nature Communications

Fig. 5: Chlamydia and HPV E6E7 coinfection regulates MMR distinctly at transcriptional and translational levels.

From: Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogramming

Fig. 5: Chlamydia and HPV E6E7 coinfection regulates MMR distinctly at transcriptional and translational levels.

hCEcto and hCEcto E6E7 2D stem cells were infected with Ctr for 48 h with or without additional treatment with Nutlin-3a (10 µM) or Lactacystin (10 µM) from 2hpi or 24hpi with MG-132 (5 µM). a, b Shown is the relative mRNA expression of MLH1 (a) and MSH6 (b) analyzed by qRT-PCR from hCEcto cells. Data represent mean ± SD of three replicates normalized to uninfected controls from a representative of two independent experiments. Statistical significance was calculated using two-sided t test, P-values are indicated; ns, no significance. (c) Immunoblot analysis of lysates from hCEcto cells for indicated proteins and Chlamydia HSP60 and β-actin as a loading control. d, e Shown is the relative mRNA expression of MLH1 (d) and MSH6 (e) analyzed by qRT-PCR from hCEcto E6E7 cells. Data represent mean ± SD of three replicates normalized to uninfected controls from a representative of two independent experiments. Statistical significance was calculated using two-sided t test, P-values are indicated; ns, no significance. f Immunoblot analysis of lysates from hCEcto E6E7 cells for indicated proteins and Chlamydia HSP60 and β-actin as a loading control. Data from (c) and (f) represent two biological replicates. g Model depicting the mechanistic regulation of MMR by HPV E6E7 and Chlamydia infections. Source data are provided as a Source Data file.

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