Fig. 3: ASF1 promotes NHEJ through its interaction with RIF1 and histone chaperone activity. | Nature Communications

Fig. 3: ASF1 promotes NHEJ through its interaction with RIF1 and histone chaperone activity.

From: RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity

Fig. 3: ASF1 promotes NHEJ through its interaction with RIF1 and histone chaperone activity.The alternative text for this image may have been generated using AI.

a, c, e, g, h Etoposide- or ICRF193-sensitivity assays of various groups of DT40 cells. The mean and s.d. from three independent experiments are shown. b, d, f Random integration assays of various groups of DT40 cells. Data are expressed as the mean number of puromycin-resistant colonies ± s.d.; n = 7, 4, 7, 3, and 3 replicates from left to right in (b); n = 3 replicates in (d, f). ns, P > 0.05; **P < 0.01; ****P < 0.0001. Statistical analysis was performed using two-way t test (a, g) or one-way ANOVA (b, d, f). Statistical source data, including the precise P values, are provided in the source data. i Cartoon showing the genetic relationships of 53BP1, RIF1, Shieldin, and ASF1.

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