Fig. 3: Overexpression of the AP-4 vesicle transport adaptor RUSC2 drives DAGLB to the cell periphery.
From: AP-4-mediated axonal transport controls endocannabinoid production in neurons
Widefield imaging of HeLa cells stably expressing RUSC2-GFP (green), labelled with anti-DAGLB (red). Top panel, wild-type; middle panel, AP4B1 knockout; lower panel, AP4B1 knockout with transient expression of AP4B1 (rescue). In the merged image, DAPI labelling of the nucleus is also shown (blue). The insets show accumulation of RUSC2-GFP-positive and DAGLB-positive puncta at the cell periphery, and this only occurred in the presence of AP-4 (wild-type and rescue). Images are representative of at least 20 images per condition, including three independent rescue transfections. Immunofluorescence and microscopy were performed independently from two technical replicates of separate coverslips from the same batch of cells. Consistent results were observed for cells expressing RUSC2 with an N-terminal GFP tag and with AP4E1 knockout (data available at https://zenodo.org/record/5696988). Scale bar: 10 µm.
