Fig. 7: Rhbdf2 signaling is obbligato for the protective effect of Trim31 on suppression of liver steatosis and inflammation. | Nature Communications

Fig. 7: Rhbdf2 signaling is obbligato for the protective effect of Trim31 on suppression of liver steatosis and inflammation.

From: The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes

Fig. 7: Rhbdf2 signaling is obbligato for the protective effect of Trim31 on suppression of liver steatosis and inflammation.

a Representative immunoblotting bands of Trim31, Rhbdf2, and corresponding downstream event indicator levels of total and phosphorylated ADAM17, MAP3K7, p-MAP3K7, NF-κB, p-NF-κB, IκBα, p-IκBα, JNK1/2, and p-JNK1/2 in liver samples isolated from the indicated mice fed a HFD for 16 weeks. b, c Records for body weight, liver weight, the ratio of liver weight/body weight (%) (b), and fasting blood glucose levels, fasting insulin levels, and HOMA-IR index (c) in a 16-week HFD-fed indicated mice (n = 10 mice per group) (**P < 0.01 vs. Flox HFD groups, ##P < 0.01 vs. THKO HFD groups). d Representative pictures for Oil red O staining and H&E staining-indicated liver histopathologic changes of a 16-week HFD-fed indicated mice (magnification, ×100; n = 10 images per group for each staining) (**P < 0.01 vs. Flox HFD groups, ##P < 0.01 vs. THKO HFD groups). e Liver lipid contents including TG, TC, and NEFA of the indicated mice after a 16-week HFD ingestion (n = 10 mice per group) (**P < 0.01 vs. Flox HFD groups, ##P < 0.01 vs. THKO HFD groups). f qPCR analysis of the relative mRNA expression of genes in the livers associated with inflammation signaling in 16-week HFD-fed mice (n = 10 liver samples per group) (*P < 0.05 vs. Flox HFD groups, #P < 0.05 vs. THKO HFD groups). g Representative images of Oil red O staining of primary hepatocytes that were transfected with AdshRhbdf2 or AdshTrim31 or co-treated with AdshRhbdf2/AdshTrim31 or PA for 10 h (magnification, ×200; n = 10 images per group for each staining) (**P < 0.01 vs. Flox HFD groups, ##P < 0.01 vs. THKO HFD groups). The relevant experiments presented in this part were performed independently at least three times. Data are expressed as mean ± SEM. The relevant experiments presented in this part were performed independently at least three times. Significance determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test.

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