Fig. 2: Preprocessing, consistency, and categorization of FRSs. | Nature Communications

Fig. 2: Preprocessing, consistency, and categorization of FRSs.

From: Massively parallel reporter perturbation assays uncover temporal regulatory architecture during neural differentiation

Fig. 2

a Illustration of the four filters applied to perturbed sequences to remove inactive and non-functional sites, both at each timepoint and across timepoints (error bars represent mean ± 1 SD; “Methods”). b Number of sequences that passed all three filters for each perturbation method. c Definition of main and sub-categories of motif binding effects based on their effect on transcription. d Distribution of categories across FRSs that pass the four filters and are under the same main category (activating or dampening) in perturbation method 3 and at least one of the perturbation methods 1 or 2. The distribution is shown across FRSs (top) and across the unique motifs and regions composing the FRSs in this study (bottom).

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