Fig. 5: Double perturbation scheme.

a Experimental design for perturbing two single motifs separately and then a double perturbation of both simultaneously, and the requirements for being included in downstream analysis. b Volcano plot for the model testing for log-additivity of the individual effects. c–g Examples of double perturbation results demonstrating different patterns of cooperation: log-additive effects consistent with a billboard model (c); log-additive effects of one dampening and one activating element (d); fully dependent cooperation consistent with the enhanceosome model (e); a billboard-enhanceosome hybrid model with one required element and one with a dampening effect (f); a redundancy example, perturbing either motif has negligible effect, but perturbing both has a substantial effect (g). All coordinates are hg19. Line plots similar to Fig. 3, mean activity ± 1 SD across the three replicates. Source data are provided as a Source Data file.