Fig. 1: Imp7 nuclear accumulation is regulated by cell density.
From: Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP
a Schematic representation of the experimental design to identify proteins sensitive to changes in nucleo-cytoplasmic balance as a function of cell density by a quantitative MS approach. Created with Biorender.com. b Quantitative MS hypothesis-free analysis of nuclear and cytosolic fractions of RPE-1 cells grown at low (10,417 cells/cm2) or high (218,750 cells/cm2) confluence. For each protein identified, the difference of cytosolic change between low and high confluence was substracted to the difference of nuclear change between low and high confluence (z-score). We selected and sorted the 5% identified proteins with the higher and lower z-scores. c, d Immunofluorescence of endogenous Imp7 in RPE-1 cells plated at the high and low confluence. Quantification is shown in graph (d). N = 58 cells for each condition, from 3 independent experiments. P-value = 1.100e−14. e–i Immunofluorescence of the indicated endogenous proteins in RPE-1 cells plated at high and low cell confluence. Rb: retinoblastoma. Data representative from three biologically independent experiments. j Quantification of the nucleo-cytoplasmic intensity ratio of the indicated proteins in high and low-density cell cultures (high and low). From left to right in the graph, N = 248, 242, 222, 144, 401, 310, 266, 209, 80, and 63 cells per condition, from 3 independent experiments. Statistical analysis with a two-tailed unpaired t-test. From left to right, P-values = 0.052, 0.706, 0.067, 0.024, and 0.131. Raw data are available in the Source Data file. Data represent mean ± s.e.m. Scale bar 10 µm. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.
