Fig. 8: YAP determines Imp7 mechanoresponse.

a, b Immunofluorescence of endogenous Imp7 and nuclei in RPE-1 cells growing at the low confluence and silenced for YAP with two independent siRNAs. Vehicle (DMSO) or Cyt D (1 µM, 1 h) treated sets are shown. Quantification is shown in graph (b). From left to right in the graph, N = 240, 236, 270, 321, 167, and 190 cells per condition, from 3 independent experiments. P-value = 1.069e−14 (DMSO siControl vs DMSO siYAP_a), 1.210e-16 (DMSO siControl vs. DMSO siYAP_b), 5.361e-12 (DMSO siControl vs. Cyt D siYAP_a), 0.101 (DMSO siYAP_a vs. DMSO siYAP_b), 0.206 (DMSO siYAP_a vs. Cyt D siControl), 0.090 (DMSO siYAP_a vs. CytD siYAP_a), 0.011 (DMSO siYAP_b vs. Cyt D siControl), 0.831 (DMSO siYAP_b vs. Cyt D siYAP_b), and 0.804 (Cyt D siYAP_a vs. Cyt D siYAP_b). c, d Immunofluorescence of endogenous Imp7 and nuclei in RPE-1 cells growing at low or high confluence treated with 6 µM XMU-MP-1 for 16 h. Quantification is shown in graph (d). From left to right in the graph, N = 93, 273, and 194 cells per condition, from 3 independent experiments. P-value = 5.480e−14 (1st vs. 2nd bars), 0.0036 (1st vs. 3rd bars), and 1.422e−25 (2nd vs. 3rd bars). e, f Immunofluorescence of endogenous Imp7 in RPE-1 cells growing at a low confluence, silenced for Smad3 and treated with (+) or without (−) TGFβ (10 ng/ml for 2 h). Quantification is shown in graph (f). From left to right in the graph, N = 51, 50, 53, and 45 cells per condition, from 3 independent experiments. g Immunoblot showing specific depletion of Smad3. Several loading controls are shown. Representative of three biologically independent experiments. Raw data are available in the Source Data file. Statistical analysis with a two-tailed unpaired t-test. Data represent mean ± s.e.m. Scale bar 10 µm. ns non-significant. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.