Fig. 1: Optofluidic single-particle detection platform.
From: Fast custom wavelet analysis technique for single molecule detection and identification
a Schematic of the experimental setup with a photo of comparison of fabricated ARROW chip with a nickel coin. The chip layout offers two options: single-peak or multi-peak fluorescence signals by coupling optical fiber into single-mode or multi-mode interference (MMI) waveguides, respectively. b Microscope image of detection region excited at 556 nm in the single-mode waveguide and 633 nm in the MMI waveguide which generates seven distinct spots in the analyte channel (analyte channel contains quantum dot-DI water solution for visualization of the excitation patterns as indicated by the red and green overlay colors). An example of target particles flowing across the MMI pattern and creating an intensity-modulated temporal signal in the collection waveguide is shown. c Examples of generated fluorescence signals: Single-peak signal is taken from fluorescent nanobeads and the multi-peak signal is generated by a single fluorescently tagged nucleic acid from a Klebsiella pneumoniae carbapenemase (KPC) bacterium. Areas under curves are shaded with the same color used in the overlay for visualization purposes.
