Fig. 7: 15N-1H HSQC spectrometric characterization of benzodiazepine compounds enhancing the binding affinity of p53p for N-MdmX.

a The perturbation of the 15N-1H HSQC resonances of the W23p53p side chain by four benzodiazepine compounds are compared. Protein and ligand ratios: black, 1:0; green, 1:0.5; blue, 1:1; yellow, 1:1.5 and red, 1:2. b, c The perturbation of the 15N–1H HSQC resonances of the W23p53p side chain by five pharmacophores are compared. The protein and ligand ratios: black, 1:0; green, 1:0.5; blue, 1:1; yellow, 1:1.5; red, 1:2, orange, 1:3 and purple, 1:5.