Fig. 1: Chd8+/− mice show abnormalities in the brain and intestine and ASD-like behaviors.
a Chd8 mutant construction and the sequences of wild-type and mutant alleles. b Full-length CHD8 protein expression in the whole brain and the gut of the mice at embryonic day 14.5 (E14.5) as well as in the hypothalamus of the mice at week 12. n = 8, 4, 7,4, 6 and 6 mice, respectively. c Weights of the whole brain and cerebrum of 12-week-old mice. n = 26, 24, 12, and 8 mice, respectively. d Lengths of the small intestine (SI) and colon of 12-week-old mice. n = 7 and 8 mice, respectively. e Body weights of the mice at weeks 4, 12, and 18. n = 20 (Week 4/Chd8+/+), 23 (Week 4/Chd8+/−), 12 (Week 12/Chd8+/+), 11 (Week 12/Chd8+/−), 14 (Week 18/Chd8+/+), and 13 (Week 18/Chd8+/−) mice, respectively. f Percentages of interaction time (left) and the preference index (right) in the social novelty preference session of the three-chamber social interaction test. n = 24 and 20 mice, respectively. g Representative traces (left) and the percentages of time in the center in the open-field test (right). n = 26 and 24 mice, respectively. h Percentages of time spent in the light box in the light/dark box test. n = 26 and 22 mice, respectively. i Percentages of interaction time (left) and the preference index (right) in the novel object recognition test. Nov novel object, Old old object. n = 25 and 24 mice, respectively. Source data are provided as a Source Data file. Quantitative data are shown as the mean ± SEM. Statistical analysis was determined by the two-tailed Mann–Whitney test (b, c, d, f (right panel), g, h and i (right panel)) and two-way ANOVA with two-tailed Turkey’s test for multiple comparisons (e, f (left panel) and i (left panel)). Significance was indicated by P value. n.s. means no significant difference.
