Fig. 5: B. uniformis reduces the expression of intestinal amino acid transporters in the Chd8+/− mice.
a Serum levels of L-glutamine in of 12-week-old mice detected by targeted metabolomics assays. n = 18, 18, and 11 mice, respectively. b–d Bulk RNA-seq analysis of the small intestine of 12-week-old mice. There were n = 3, n = 3, and n = 5 mice in the KN, KB, and WN groups, respectively (WN: Chd8+/+ mice gavaged with PBS; KN: Chd8+/− mice gavaged with PBS; KB: Chd8+/− mice gavaged with B. uniformis). b Venn diagram illustrating the count of DE genes between KN_WN and DE genes between KB_WN. The DE genes were determined under a strict threshold of adjusted P < 0.01 and |log2(cf)| > 0.585. c Euclidean distance of KB_KN (Chd8+/− mice gavaged with PBS and Chd8+/− mice gavaged with B. uniformis), KB_WN, and KN_WN. The two-tailed Mann–Whitney test was used to calculate the significance. Box plots were based on 350 data points and showed center line as median, box limits as upper and lower quartiles, whiskers as 1.5 × interquartile range and dots as outliers. d GSEA plot shows the enrichment of the amino acid transmembrane transporter activity pathway in small intestinal cells of the Chd8+/− mice gavaged with PBS compared to the Chd8+/− mice gavaged with B. uniformis. e Immunofluorescence staining of SLC6A19 and SLC7A8 in the ileum of the mice at week 12. Scale bar, 20 μm. n = 3 mice per group. Each symbol represents one image; seven to nine images per mouse. n = 28, 26, and 21 symbols, respectively. f Western blotting analysis of the SLC6A19 and SLC7A8 protein levels in the small intestine of the mice at week 12. n = 9, 7, and 11 mice, respectively. Quantitative data are shown as the mean ± SEM. Statistical analysis was determined by one-way ANOVA with two-tailed Tukey’s multiple comparison test (a, e, f). Significance was indicated by P value.
