Fig. 1: Targeting Mat1a reverses diet-induced obesity. | Nature Communications

Fig. 1: Targeting Mat1a reverses diet-induced obesity.

From: Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis

Fig. 1: Targeting Mat1a reverses diet-induced obesity.The alternative text for this image may have been generated using AI.

Two-month-old C57BL/6J mice were fed a chow-diet (CD) or a high-fat diet (HFD) for 10 weeks. During the last 4 weeks mice were treated with Mat1a antisense oligonucleotide (ASO) or control ASO (25 mg/kg/week) until sacrifice. a Knockdown of Mat1a ASO. MATI/III and transferrin blots and densitometries in liver of CD- (n = 6) and HFD-fed control (n = 7) and Mat1a (n = 8) ASO-treated mice. b Representative photograph of HFD-fed ASO-treated mice. c Body weight change for CD- (n = 6) and HFD-fed control (n = 7) and Mat1a (n = 8) ASO-treated mice. d Body composition in HFD-fed control (n = 7) and Mat1a (n = 7) ASO-treated mice. e Food intake for CD- (n = 2 cages) and HFD-fed control (n = 2 cages) and Mat1a (n = 2 cages) ASO-treated mice. f Glucose tolerance test (GTT), insulin serum levels and g insulin tolerance test (ITT) of CD- (n = 5) and HFD-fed control (n = 7) and Mat1a (n = 7) ASO-treated mice at the third week of treatment. Data are also indicated as area under the curve (AUC) expressed in arbitrary units (A.U.). h Dark-phase energy balance, locomotor activity, and light phase energy balance in HFD-fed control (n = 6 in light and dark phase) and Mat1a (n = 5 in light and n = 6 in dark phase) ASO-treated mice housed in metabolic cages over 2 days at the end of the treatment period. i Overall energy balance and locomotor activity in thermoneutrality in HFD-fed control (n = 7) and Mat1a (n = 6) ASO-treated mice housed in metabolic cages over 2 days at the end of the treatment period. Values are means ± SEM for time course representations, and means ± SD for histograms. Statistically significant differences are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001 for Control ASO HFD vs. Mat1a ASO HFD; and #p < 0.05, ##p < 0.01, and ###p < 0.001 for Control ASO CD vs. Control ASO HFD (two-tailed Student’s test). Statistical analysis performed by ANOVA test comparing Control ASO CD vs. Control ASO HFD; Control ASO HFD vs. Mat1a ASO HFD; Control ASO CD vs. Mat1a ASO HFD is presented in GTT and ITT curves. Statistical analysis for energy expenditure was performed by two-way ANCOVA test. Source data are provided as a Source data file.

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