Fig. 1: bFGF undergoes phase separation in the presence of PEG or heparin.
From: Phase separation on cell surface facilitates bFGF signal transduction with heparan sulphate
a Confocal microscopy images of assembling status of 5 μM eGFP-bFGF with 0–20% PEG, with eGFP alone in 10% PEG as control. Scale bar = 5 µm. b Turbidity measurement of eGFP-bFGF with 0–20% PEG. n = 3 biologically independent samples, data are presented as mean values ± SEM. Comparisons among groups were performed using ordinary one-way ANOVA test. c, FRAP of the droplets formed by eGFP-bFGF with 10% PEG. n = 5 biologically independent samples, data are presented as mean values ± SEM. d FRAP of the aggregates formed by eGFP-bFGF with 20% PEG. n = 3 biologically independent samples, data are presented as mean values ± SEM. e Confocal microscopy images of assembling status of 5 µM eGFP-bFGF mixed with heparin at different ratios, with heparin: eGFP = 1:20 as control. Scale bar = 5 µm. f Turbidity measurement of eGFP-bFGF with heparin. n = 3 biologically independent samples, data are presented as mean values ± SEM. g FRAP results of the droplets formed by heparin:eGFP-bFGF = 1:20. n = 3 biologically independent samples, data are presented as mean values ± SEM. h Confocal microscopy images of assembling status of 5 uM eGFP-bFGF mixed with de-O-sulphated heparin at different ratios. Scale bar = 5 µm.
