Fig. 2: Interaction of bFGF and heparin.
From: Phase separation on cell surface facilitates bFGF signal transduction with heparan sulphate
a Confocal microscopy images of bFGF condensates with various ratios of heparin with 5 mM DTT or 1 mM H2O2. Scale bar = 5 µm. b FRAP of the condensates formed by eGFP-bFGF oxidised in the presence of 1 mM peroxide. n = 3 biologically independent samples, data are presented as mean values ± SEM. c FRAP of the condensates formed by oxidised eGFP-bFGF: heparin = 1:20. n = 4 biologically independent samples, data are presented as mean values ± SEM. d Non-reduced SDS-PAGE of the eGFP-bFGF with different oxidative conditions and heparin concentrations, showing its assembling status. e Schematic illustration of the proposed mechanism of bFGF assembling under different oxidative status with heparin/ de-O-sulphated heparin.
