Fig. 3: bFGF phase separates on cell surface with heparan sulphate.
From: Phase separation on cell surface facilitates bFGF signal transduction with heparan sulphate
a Z-stack scanning of droplets formation on the cell surface. N2a, CHO-K1 or MEF cells incubated with eGFP-bFGF were imaged under confocal microscope with the Z-stack method, showing droplets condensation on the cell surface. Scale bar = 5 µm. b FRAP of the condensates formed by eGFP-bFGF. n = 4, 3 and 6 biologically independent samples for N2a, CHO-K1 and MEF cells respectively, data are presented as mean values ± SEM. c Confocal microscopy images of heparan sulphate (HS, magenta) immunostaining and eGFP-bFGF (green) on N2a, CHO-K1 and MEF cells. Scale bar = 5 µm. d Confocal microscopy images of eGFP-bFGF phase separation on MEF cell surface with or without treatment with heparinase III. Scale bar = 10 µm. e Confocal microscopy images of eGFP-bFGF phase separation on the surface of wildtype CHO-K1 and the HS deficient CHO-677 cells. Scale bar = 10 µm.
