Fig. 2: ADAP1 is expressed in primary human CD4⁺ T cells.

a Schematic of primary CD4⁺ T cell states generated and analyzed in the subsequent panels. b Relative mean ± s.d. (n = 3) mRNA expression of ADAP1 in one representative donor across differing T cell states (repeated in two other donors with similar results). T_N were sampled on the day of isolation, T_M were sampled 18–21 days post donor isolation depending on when they reached quiescence, T_E and T_M+Stim were samples stimulated for 24 h. (one-way ANOVA followed by Dunnett’s test for multiple comparisons to mock). c Representative western blot analysis of ADAP1 expression in primary CD4⁺ T cells across differing states. T_N were sampled on the day of isolation, T_M were sampled 18–21 days post isolation depending on when they reached quiescence, T_E and T_M+Stim were samples stimulated for 24 h (repeated in two other donors with similar results). d Relative mean ± s.e.m. (n = 3) mRNA expression of ADAP1 in two representative donors across differing times post-stimulation. e Representative western blot analysis of ADAP1 expression in two representative donors across differing times post-stimulation. Source data are provided as a Source Data file.