Fig. 3: Loss of ADAP1 hinders latent HIV-1 reactivation ex vivo. | Nature Communications

Fig. 3: Loss of ADAP1 hinders latent HIV-1 reactivation ex vivo.

From: ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS–ERK–AP-1 T cell signaling-transcriptional axis

Fig. 3: Loss of ADAP1 hinders latent HIV-1 reactivation ex vivo.

a Schematic of generation of ex vivo model of HIV-1 latency and CRISPR-Cas9 engineered cells HIV-CtrlCRISPR, HIV-CXCR4CRISPR, HIV-NF-κBCRISPR(p65 subunit), and HIV-ADAP1CRISPR. b Representative western blot analysis of CRISPR-Cas9-mediated knockout efficiency (repeated in two other donors with similar results). c Luciferase analysis of HIV-CtrlCRISPR, HIV-CXCR4CRISPR, HIV-NFκBCRISPR, and HIV-ADAP1CRISPR TM stimulated for 48 h with PMA. Data represents mean ± s.d. fold change of three donors (n = 3 each), HIV-CtrlCRISPR normalized to 1 representing max activation. (one-way ANOVA followed by Dunnett’s test for multiple comparison to CtrlCRISPR). ns not significant. Source data are provided as a Source Data file.

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