Fig. 6: ADAP1 promotes ERK–AP-1 activation in a KRAS-dependent manner.
a Jurkat cell lines expressing Strep-tagged ADAP1 or GFP were electroporated with TF-responsive element driving Firefly luciferase reporters. Firefly luciferase activity was measured 48 h post electroporation normalized to a CMV-Renilla control. Data represents mean ± s.d. of three independent electroporation experiments in triplicate (two-way ANOVA followed by Sidak’s test for multiple comparison between ADAP1− and ADAP1+ groups). ns not significant. b Western blot analysis of Jurkat cell lines expressing Strep-tagged ADAP1 or GFP in the absence of stimulation. Blots are representative of three independent experiments. c Fold luciferase activity comparing RAS family members in transduced Jkt-HIVLuc model. Data represents mean ± s.d. fold luciferase activity (n = 3). (one-way ANOVA followed by Dunnett’s test for multiple comparisons to mock). ns not significant. d Western blot analysis of Jurkat cells co-transduced with ADAP1 (WT, GAP Mut, or PH1 Mut) and limiting amounts of Flag-tagged KRAS (WT or dominant negative S17N). Blots are representative of three independent experiments. e Fold luciferase activity comparing co-expression of ADAP1 (WT, GAP Mut or PH1 Mut) with low level (4:1 lentiviral dilution) KRAS (WT or DN) in transduced Jkt-HIVLuc cells. Data represents mean ± s.d. fold luciferase activity (n = 3) (two-way ANOVA followed by Dunnett’s test for multiple comparison to mock). ns not significant. f Western blot analysis of primary ADAP1CRISPR and CtrlCRISPR TM stimulated with anti-TCR/anti-CD28 beads in a time-course-dependent manner from 0 to 120 min. Blots shown are representative examples from one of four donors. Source data are provided as a Source Data file.
