Fig. 4: Claudin-5 but not VEGFR2 is involved in Unc5B BBB regulation.
From: Endothelial Unc5B controls blood-brain barrier integrity
a Unc5B deletion and eGFP::Claudin-5 gene overexpression strategy using tamoxifen injection. b Quantification of P67 brain cadaverine content, 30 min after i.v cadaverine injection, n = 4 Unc5BiECko and n = 4 Unc5BiECko;eGFP::Claudin-5 brains. Each dot represents one mouse. Western blot (c) and quantification (d) of P67 brain protein extracts, n = 7 Unc5Bfl/fl and n = 8 Unc5BiECko brains. Each dot represents one mouse. One control mouse was set as 1. e Unc5B and VEGFR2-Y949F gene recombination strategy using tamoxifen injection. f Quantification of P67 brain cadaverine content, 30 min after i.v cadaverine injection, n = 4 Unc5Bfl/fl;Y949F and n = 6 Unc5BiECko;Y949F brains. Each dot represents one mouse. Western blot (g) and quantification (h) of brain protein extracts, n = 7 Unc5Bfl/fl and n = 8 Unc5BiECko brains. Each dot represents one mouse. One control mouse was set as 1. Immunofluorescence with the indicated antibodies (i) and quantification (j) of VE-cadherin coverage on P67 brain sections. Each dot is the mean of several images, n = 3 Unc5Bfl/fl and n = 5 Unc5BiECko brains. One control mouse was set as 1. All data are shown as mean ± SEM. NS non-significant. Two-sided Mann–Whitney U test was performed for statistical analysis. Source data are provided as a Source Data file.
