Fig. 4: Loss of SOX10 induces a dormant/quiescent phenotype in vivo.
From: Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma
a Average tumor volume ±SEM for MeWo parental and SOX10 knockout clones #2.1 and #4.11. Number of mice per cohort as indicated in the figure. b Average Luciferase signal (Avg Radiance [p/s/cm²/sr]) ±SEM for MeWo parental and SOX10 knockout clones #2.1 and #4.11. Number of mice per cohort as indicated in the figure. c Luciferase signal in MeWo #2.1 (2 mice) and #4.11 (2 mice) 90 days after injection. Mice injected with parental MeWo cells did not survive 90 days; hence, their exclusion from the 90 days imaging. d IHC comparing p21Cip1 and Ki67 expression in MeWo parental versus SOX10 knockout (clones #2.1 and #4.11) tumors collected at the end of the experiment (day 35). Shown is the mean ± SD from three independent tumors generated either from parental or SOX10 knockout MeWo cells. Scale bar, 200 μm. p-values were calculate using two-sided t-test. e Representative images showing second-harmonic generation signatures from three independent tumors from MeWo parental or SOX10 knockout xenografts. Images shown correspond to reconstituted monochromatic images pseudocolored according to “intensity heat-maps” of total second-harmonic generation signal. Warmer tones indicate higher second-harmonic generation signals (color tone bar is provided). Scale bars, 50 μm. f Quantitative analysis of polymerized collagen signatures from images in e. Three independent tumors generated either from parental or SOX10 knockout MeWo cells were analyzed. In total, 12 images from parental and 7 images from SOX10 knockout cells tumors were assessed. Values presented are normalized to MeWo parental xenograft. Data are expressed as mean ± SD. p = 0.025. p-values were calculated using the Wilcoxon two-sample test.
