Fig. 1: Raindrop- and mechanical stimuli (MS)-induced transcriptome profiles highly correlate with each other. | Nature Communications

Fig. 1: Raindrop- and mechanical stimuli (MS)-induced transcriptome profiles highly correlate with each other.

From: Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Fig. 1

a Enriched Gene Ontology (GO) categories of 1050 raindrop (10 falling droplets)-induced genes in the wild type (Col-0) 15 min after treatment. The top 10 categories are shown in ascending order of P values (one-sided hypergeometric test). b, c Transcript levels of MS-induced and defense-related genes in 4-week-old Col-0 plants 15 min after being treated with 10 falling droplets (raindrop, b) or 1 brushing (MS, c), determined by RT-qPCR and normalized to UBIQUITIN 5 (UBQ5). Data are presented as mean ± SD. n = 6 plants examined over three independent experiments. Each dot indicates a technical replicate. d Enriched GO categories of 1241 MS (1 brushing)-induced genes in Col-0 15 min after treatment. The top ten categories are shown in ascending order of P values (one-sided hypergeometric test). e Venn diagram of the overlap between transcriptome datasets from raindrop- and MS-induced genes (likelihood ratio test; P < 0.05). f Radar chart of intensity compared with mock (log2FC) and Pearson correlation coefficient (r = 0.917288423) of 917 raindrop- and MS-induced genes (left). Intensities of major immune regulator genes induced by raindrops and MS in RNA-seq analysis (log2FC) (likelihood ratio test; P < 0.05) (right).

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