Fig. 3: M6A stacking on a helix end stabilizes secondary structure as compared to A stacking.
From: Secondary structure prediction for RNA sequences including N6-methyladenosine
The ΔΔG°37 (kcal/mol) for dangling ends and terminal mismatches as a result of N6-methylation (Supplementary Table S4) is shown, where negative values mean greater folding stability for m6A than A. The motifs shown here have a terminal base pair (left side of motif), and either a dangling end or terminal mismatch right (right side of motif). On average, the methylated motifs are more stabilizing than the unmethylated motifs, although the extent of the stabilization is sequence dependent. Uncertainty estimates are propagated from the uncertainty from the individual optical melting experiments (see “Error propagation” in the “Methods”).
