Fig. 4: The Gi engagement of BLT1.
From: Structural basis of leukotriene B4 receptor 1 activation
a The interaction between the receptor and the αH5, viewing from the TM5, TM6, TM7-H8 front angle. b The interaction between the receptor and Gαi, viewing from the TM3, TM4, and ICL2 front angle. c A detailed analysis of the F123ICL2/Gαi interaction. d The insertion of F123ICL2 into the hydrophobic cavity of Gαi. The light orange color indicates hydrophobicity. e An alignment of BLT1 with lipid receptors around the F123ICL2 region. f A SRE reporter assay to analyze the contribution of key residues in the Gi engagement. LTB4, 300 nM; data are presented as mean values ± SD; n = 3 independent samples; n.s. no significant; *p < 0.05; **p < 0.01; ***p < 0.001. The exact p value for T53A, D114A, R115A, F123A, T130A, are: 0.0001, 0.0001, 0.0002, 0.0001, 0.0001, respectively. T-test, two tailed, sample equal variance.
