Fig. 1: PRX62 associates with enhanced RH growth under low temperature condition.

a Representative accessions of A. thaliana showing short (Rd-0 and Te-0) and long root hair (RH) phenotypes (Col-0, Sf-2, and Wc-1) when grown at low temperature (10 °C). Scale bars 500 µm b Manhattan plots for RH length at 22 °C (top plot) and at 10 °C (bottom plot). Coarse analysis was performed using GWAPP²⁹ (https://gwapp.gmi.oeaw.ac.at/). Arabidopsis chromosomes are depicted in different colors. The red box in the bottom plot indicates the genomic region significantly associated with root hair length at 10 °C. c Zoomed-in of the genomic region red-boxed in (b). The lead SNP (m190905) and three additional SNPs highly associated with RH length localize within PRX62 (AT5G39580). PRX62 splice variants (AT5G39580.1, AT5G39580.2) are depicted. The four associated SNPs (in red) are in high linkage disequilibrium (LD) with each other, and they are combined into two major and opposite haplotypes in the population (ACAG and TTCA). LD plot as heat-map and values for LD (coefficient of correlation, r²) are shown. PRX62 gene structure showing two splice variants (isoforms), common haplotypes, and type of mutation for AT5G39580.1/ AT5G39580.2 are indicated above the Manhattan plot. The SNP m190904 is a non-synonymous SNP for AT5G39580.2 causing a change from Glutamic Acid to Lysine at position 200 (E200K) in the amino acid sequence. This same SNP is synonymous (sSNP) for the full-length isoform AT5G39580.1. d The full-length variant of PRX62 (AT5G39580.1) is upregulated at low temperature (10 °C) while the shorter variant (AT5G39580.2) is almost not detected. Expression measured by RNA-seq of PRX62. TPM = Transcripts Per Kilobase Million.